pgc1α antibodies (Novus Biologicals)
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Novus Biologicals
pgc1α antibodies
Pgc1α Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pgc1%CE%B1/PGC1+alpha+Antibody+%5BDyLight+405%5D/us12571805-519-11-21
Average 94 stars, based on 2 article reviews
Pgc1α Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pgc1%CE%B1/PGC1+alpha+Antibody+%5BDyLight+405%5D/us12571805-519-11-21
Average 94 stars, based on 2 article reviews
pgc1α antibodies - by Bioz Stars,
2026-10
94/100 stars
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Western Blot:Article Title: Chaperone-mediated autophagy manipulates PGC1α stability and governs energy metabolism under thermal stress. Article Snippet: After separating equal amounts of total protein by SDSPAGE gels, the proteins were transferred to a PVDF membrane (Millipore) and blocked with 5% milk (fatty-acid free) for 1 h at room temperature. .. Followed primary antibodies: Anti-UCP1 (Abcam,ab10983, 1:1000), anti-Hsc70 (Abcam, ab51052, 1:1000), total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, 1:250), anti-PPARα/NR1C1 (novus, NB300-537, 1:1000), anti-PPARγ (CST, 2435, 1:1000), anti-LAMP-2A (Invitrogen, 51-2200, 1:1000), anti-PGC1α (novus, NBP1-04676, 1:1000), anti-CPT-1α (novus, NB100-53791, 1:1000), anti-CPT1β (ABclonal, A6796, 1:1000), anti-Flag (ABclonal, AE005, 1:2000), anti-DJ-1/PARK7 (ABclonal, A19097, 1:1000), anti-βtubulin (Cowin Biotech, CW0098M, 1:2000), anti-HA (Proteintech, 51064-2-AP, 1:2000), anti-V5(CST, 13202, 1:2000), anti-LAMP2a (Abmart, T55518, 1:1000) were incubated with the membranes, which were then washed three times in TBST (Tris-buffered saline containing 0.1% Tween 20) before visualization using the ECL. .. Western blot images were analyzed for densitometry using ImageJ (National Institutes of Health)58. Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia Article Snippet: .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia. Article Snippet: The secondary antibodies used were goat anti-rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo Fisher, SA5-10010, 1:400 dilution) or goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 (Thermo Fisher, A21070; 1:500 dilution) for 1 h at room temperature. .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: Incubation:Article Title: Chaperone-mediated autophagy manipulates PGC1α stability and governs energy metabolism under thermal stress. Article Snippet: After separating equal amounts of total protein by SDSPAGE gels, the proteins were transferred to a PVDF membrane (Millipore) and blocked with 5% milk (fatty-acid free) for 1 h at room temperature. .. Followed primary antibodies: Anti-UCP1 (Abcam,ab10983, 1:1000), anti-Hsc70 (Abcam, ab51052, 1:1000), total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, 1:250), anti-PPARα/NR1C1 (novus, NB300-537, 1:1000), anti-PPARγ (CST, 2435, 1:1000), anti-LAMP-2A (Invitrogen, 51-2200, 1:1000), Saline:Article Title: Chaperone-mediated autophagy manipulates PGC1α stability and governs energy metabolism under thermal stress. Article Snippet: After separating equal amounts of total protein by SDSPAGE gels, the proteins were transferred to a PVDF membrane (Millipore) and blocked with 5% milk (fatty-acid free) for 1 h at room temperature. .. Followed primary antibodies: Anti-UCP1 (Abcam,ab10983, 1:1000), anti-Hsc70 (Abcam, ab51052, 1:1000), total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, 1:250), anti-PPARα/NR1C1 (novus, NB300-537, 1:1000), anti-PPARγ (CST, 2435, 1:1000), anti-LAMP-2A (Invitrogen, 51-2200, 1:1000), Concentration Assay:Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia Article Snippet: .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia. Article Snippet: The secondary antibodies used were goat anti-rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo Fisher, SA5-10010, 1:400 dilution) or goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 (Thermo Fisher, A21070; 1:500 dilution) for 1 h at room temperature. .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: Bradford Protein Assay:Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia Article Snippet: .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia. Article Snippet: The secondary antibodies used were goat anti-rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo Fisher, SA5-10010, 1:400 dilution) or goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 (Thermo Fisher, A21070; 1:500 dilution) for 1 h at room temperature. .. The concentration of protein was measured with Bradford protein assay solution (Bio-Red, 5000006) and the same amount of total protein was loaded in SDS–PAGE for western blotting and probed with specific antibodies: other:Article Title: Chaperone-mediated autophagy manipulates PGC1α stability and governs energy metabolism under thermal stress. Article Snippet: Western blot images were analyzed for densitometry using ImageJ (National Institutes of Health)58. |
